diff --git a/CHANGELOG.md b/CHANGELOG.md
index a6b557b9..f33a44eb 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -7,6 +7,13 @@
 
 # Changelog
 
+## Sprint 100 raster tile Segmentation Lab handoff (2026-06-24)
+
+- Added Segmentation Lab tile manifest state and wired it into the existing segmentation run request `tile_manifest_path`.
+- Added a raster inspector handoff action that fills the selected raster dataset and tile manifest path in Segmentation Lab.
+- Mirrored the existing Detection Lab manifest input pattern without adding new backend routes, migrations, AI dependencies or model behavior.
+- Added regression coverage for the segmentation handoff wiring.
+
 ## Sprint 99 raster tile Detection Lab handoff (2026-06-23)
 
 - Surfaced the latest persisted `raster.tile` manifest path in the raster dataset inspector.
diff --git a/backend/tests/test_sprint100_segmentation_manifest_handoff.py b/backend/tests/test_sprint100_segmentation_manifest_handoff.py
new file mode 100644
index 00000000..9256662f
--- /dev/null
+++ b/backend/tests/test_sprint100_segmentation_manifest_handoff.py
@@ -0,0 +1,28 @@
+from pathlib import Path
+
+
+ROOT = Path(__file__).resolve().parents[2]
+
+
+def test_raster_tile_manifest_can_handoff_to_segmentation_lab() -> None:
+    app = (ROOT / "frontend" / "src" / "App.tsx").read_text(encoding="utf-8")
+    hook = (ROOT / "frontend" / "src" / "hooks" / "useSegmentationWorkflow.ts").read_text(encoding="utf-8")
+    detail_panel = (ROOT / "frontend" / "src" / "components" / "datasets" / "DatasetDetailPanel.tsx").read_text(encoding="utf-8")
+    raster_controls = (ROOT / "frontend" / "src" / "components" / "datasets" / "RasterControls.tsx").read_text(encoding="utf-8")
+    segmentation_lab = (ROOT / "frontend" / "src" / "components" / "segmentation" / "SegmentationLab.tsx").read_text(
+        encoding="utf-8",
+    )
+
+    assert "segmentationTileManifestPath" in hook
+    assert "setSegmentationTileManifestPath" in hook
+    assert "tile_manifest_path: segmentationTileManifestPath.trim() || null" in hook
+    assert "segmentationTileManifestPath={segmentationTileManifestPath}" in app
+    assert "onSetTileManifestPath={setSegmentationTileManifestPath}" in app
+    assert "onUseTileManifestForSegmentation: useRasterTileManifestForSegmentation" in app
+    assert "setSegmentationTileManifestPath(manifestPath)" in app
+    assert "setSelectedSegmentationDatasetId(selectedDataset.id)" in app
+    assert "onUseTileManifestForSegmentation" in detail_panel
+    assert "Use in Segmentation Lab" in raster_controls
+    assert "disabled={!latestRasterTileManifestPath}" in raster_controls
+    assert "Tile manifest" in segmentation_lab
+    assert "Raster tile manifest path" in segmentation_lab
diff --git a/docs/CODEX_EXECUTION_LOG.md b/docs/CODEX_EXECUTION_LOG.md
index a5917c6d..844f8920 100644
--- a/docs/CODEX_EXECUTION_LOG.md
+++ b/docs/CODEX_EXECUTION_LOG.md
@@ -1,3 +1,34 @@
+## Sprint 100 raster tile Segmentation Lab handoff (2026-06-24)
+
+Changed:
+- Added `segmentationTileManifestPath` state to `frontend/src/hooks/useSegmentationWorkflow.ts`.
+- Sent `tile_manifest_path` in existing segmentation run requests when the field is populated.
+- Added a `Tile manifest` input to `frontend/src/components/segmentation/SegmentationLab.tsx`.
+- Added a `Use in Segmentation Lab` handoff beside the existing Detection Lab handoff in `frontend/src/components/datasets/RasterControls.tsx`.
+- Wired the handoff through `frontend/src/components/datasets/DatasetDetailPanel.tsx` and `frontend/src/App.tsx`.
+- Updated `frontend/README.md` and `CHANGELOG.md`.
+- Added `backend/tests/test_sprint100_segmentation_manifest_handoff.py`.
+
+Validation:
+- RED: `python -m pytest backend\tests\test_sprint100_segmentation_manifest_handoff.py -q` failed before implementation because segmentation manifest state and handoff wiring did not exist.
+- `python -m pytest backend\tests\test_sprint100_segmentation_manifest_handoff.py -q` passed.
+- `cd frontend && npm run typecheck` passed.
+- `python -m pytest backend\tests\test_sprint100_segmentation_manifest_handoff.py backend\tests\test_sprint99_raster_ui_handoff.py backend\tests\test_sprint88_ai_lab_density.py backend\tests\test_sprint9_segmentation_foundation.py -q` passed: 17 tests.
+- `cd frontend && npm run build` passed.
+- `python -m compileall backend/app` passed.
+- `python -m pytest -q` from `backend/` passed: 323 tests.
+- `bash scripts/run_readiness_check.sh` passed.
+- `cd backend && python -m alembic heads` passed: `202606120900 (head)`.
+- `cd backend && python -m alembic upgrade head --sql` passed.
+- `bash -n scripts/live_migration_smoke.sh` passed.
+
+Limitations:
+- Frontend handoff only; no backend route, migration, provider fetching, AI dependency or model behavior changes.
+- Segmentation remains governed by the existing model registry and explicit-run controls.
+
+Next recommended pass:
+- Run full readiness, redeploy, then validate live bundle markers and raster demo smoke on `http://192.168.10.150:1202`.
+
 ## Sprint 99 raster tile Detection Lab handoff (2026-06-23)
 
 Changed:
diff --git a/frontend/README.md b/frontend/README.md
index 6d4322b0..85f3f512 100644
--- a/frontend/README.md
+++ b/frontend/README.md
@@ -277,7 +277,7 @@ AI Lab run controls explicitly explain when no raster dataset is available, inst
 - When the Map workspace has no active result layer, it lists ready vector/GeoJSON datasets as direct quick actions so populated demo projects can jump straight from the empty state to map inspection.
 - The Data catalog shows a compact selected/reference/candidate/source summary and scan-friendly badges. Persisted `reference` datasets are shown as Reference, non-reference vector/GeoJSON layers are shown as QA Candidates for workbench scanning, and raster/other uploads remain Source.
 - Dataset cards explain the recommended next action and use compact two-line action buttons for inspect, map, export/QA and metadata refresh. Disabled actions keep a visible reason, such as `Vector/GeoJSON only`.
-- Raster controls show the latest generated tile manifest path from persisted `raster.tile` jobs and can hand that path directly to Detection Lab with the selected raster dataset.
+- Raster controls show the latest generated tile manifest path from persisted `raster.tile` jobs and can hand that path directly to Detection Lab or Segmentation Lab with the selected raster dataset.
 - The QA/QC workspace shows candidate/reference handoff cards and resolves persisted quality-check dataset IDs back to dataset names when the datasets are loaded in the current project context.
 - The QA/QC workspace includes a selected-check evidence drilldown with candidate/reference provenance, false-positive/negative metric evidence, map handoff context and parameters/findings JSON.
 
diff --git a/frontend/src/App.tsx b/frontend/src/App.tsx
index ccb73835..26b69ed5 100644
--- a/frontend/src/App.tsx
+++ b/frontend/src/App.tsx
@@ -271,6 +271,7 @@ function App(): JSX.Element {
     selectedSegmentationDatasetId,
     selectedSegmentationModelId,
     selectedSegmentationModel,
+    segmentationTileManifestPath,
     segmentationConfidenceThreshold,
     runningSegmentation,
     segmentationRunResult,
@@ -294,6 +295,7 @@ function App(): JSX.Element {
     resetSegmentationForProject,
     setSelectedSegmentationDatasetId,
     setSelectedSegmentationModelId,
+    setSegmentationTileManifestPath,
     setSegmentationConfidenceThreshold,
     setSelectedSegmentationRunId,
     setSegmentationClassFilter,
@@ -306,6 +308,15 @@ function App(): JSX.Element {
     loadProjectData,
     loadQualityChecks,
   })
+  const useRasterTileManifestForSegmentation = () => {
+    const manifestPath = latestRasterTileManifestPath.trim()
+    if (!manifestPath || !selectedDataset || selectedDataset.dataset_type !== 'raster') {
+      return
+    }
+    setSegmentationTileManifestPath(manifestPath)
+    setSelectedSegmentationDatasetId(selectedDataset.id)
+    setActiveWorkspace('ai')
+  }
   const {
     exports,
     latestExport,
@@ -859,6 +870,7 @@ function App(): JSX.Element {
                 segmentationModelError={segmentationModelError}
                 selectedSegmentationDatasetId={selectedSegmentationDatasetId}
                 selectedSegmentationModelId={selectedSegmentationModelId}
+                segmentationTileManifestPath={segmentationTileManifestPath}
                 segmentationConfidenceThreshold={segmentationConfidenceThreshold}
                 runningSegmentation={runningSegmentation}
                 segmentationRunResult={segmentationRunResult}
@@ -881,6 +893,7 @@ function App(): JSX.Element {
                 onLoadModels={loadSegmentationModels}
                 onSelectDataset={setSelectedSegmentationDatasetId}
                 onSelectModel={setSelectedSegmentationModelId}
+                onSetTileManifestPath={setSegmentationTileManifestPath}
                 onSetConfidenceThreshold={setSegmentationConfidenceThreshold}
                 onRunSegmentation={runSegmentation}
                 onLoadRuns={() => loadSegmentationRuns()}
@@ -999,6 +1012,7 @@ function App(): JSX.Element {
               onRunRasterClip: runRasterClip,
               onRunRasterTile: runRasterTile,
               onUseTileManifestForDetection: useRasterTileManifestForDetection,
+              onUseTileManifestForSegmentation: useRasterTileManifestForSegmentation,
               onRunRasterNdvi: runRasterNdvi,
               onRunRasterNdwi: runRasterNdwi,
               onRunRasterNdbi: runRasterNdbi,
diff --git a/frontend/src/components/datasets/DatasetDetailPanel.tsx b/frontend/src/components/datasets/DatasetDetailPanel.tsx
index ba7365f6..6f64a2b8 100644
--- a/frontend/src/components/datasets/DatasetDetailPanel.tsx
+++ b/frontend/src/components/datasets/DatasetDetailPanel.tsx
@@ -59,6 +59,7 @@ export interface DatasetDetailPanelProps {
   onRunRasterClip: () => void
   onRunRasterTile: () => void
   onUseTileManifestForDetection: () => void
+  onUseTileManifestForSegmentation: () => void
   onRunRasterNdvi: () => void
   onRunRasterNdwi: () => void
   onRunRasterNdbi: () => void
@@ -142,6 +143,7 @@ export function DatasetDetailPanel({
   onRunRasterClip,
   onRunRasterTile,
   onUseTileManifestForDetection,
+  onUseTileManifestForSegmentation,
   onRunRasterNdvi,
   onRunRasterNdwi,
   onRunRasterNdbi,
@@ -209,6 +211,7 @@ export function DatasetDetailPanel({
               onRunRasterClip={onRunRasterClip}
               onRunRasterTile={onRunRasterTile}
               onUseTileManifestForDetection={onUseTileManifestForDetection}
+              onUseTileManifestForSegmentation={onUseTileManifestForSegmentation}
               onRunRasterNdvi={onRunRasterNdvi}
               onRunRasterNdwi={onRunRasterNdwi}
               onRunRasterNdbi={onRunRasterNdbi}
diff --git a/frontend/src/components/datasets/RasterControls.tsx b/frontend/src/components/datasets/RasterControls.tsx
index f7d57142..8234ec93 100644
--- a/frontend/src/components/datasets/RasterControls.tsx
+++ b/frontend/src/components/datasets/RasterControls.tsx
@@ -40,6 +40,7 @@ interface RasterControlsProps {
   onRunRasterClip: () => void
   onRunRasterTile: () => void
   onUseTileManifestForDetection: () => void
+  onUseTileManifestForSegmentation: () => void
   onRunRasterNdvi: () => void
   onRunRasterNdwi: () => void
   onRunRasterNdbi: () => void
@@ -93,6 +94,7 @@ export function RasterControls({
   onRunRasterClip,
   onRunRasterTile,
   onUseTileManifestForDetection,
+  onUseTileManifestForSegmentation,
   onRunRasterNdvi,
   onRunRasterNdwi,
   onRunRasterNdbi,
@@ -168,6 +170,9 @@ export function RasterControls({
           <button type="button" onClick={onUseTileManifestForDetection} disabled={!latestRasterTileManifestPath}>
             Use in Detection Lab
           </button>
+          <button type="button" onClick={onUseTileManifestForSegmentation} disabled={!latestRasterTileManifestPath}>
+            Use in Segmentation Lab
+          </button>
         </div>
       </section>
       <section className="raster-guardrail-surface" aria-label="Processing guardrails">
diff --git a/frontend/src/components/segmentation/SegmentationLab.tsx b/frontend/src/components/segmentation/SegmentationLab.tsx
index a404c488..b8bbb02d 100644
--- a/frontend/src/components/segmentation/SegmentationLab.tsx
+++ b/frontend/src/components/segmentation/SegmentationLab.tsx
@@ -13,6 +13,7 @@ interface SegmentationLabProps {
   segmentationModelError: string | null
   selectedSegmentationDatasetId: string
   selectedSegmentationModelId: string
+  segmentationTileManifestPath: string
   segmentationConfidenceThreshold: number
   runningSegmentation: boolean
   segmentationRunResult: SegmentationRunResponse | null
@@ -35,6 +36,7 @@ interface SegmentationLabProps {
   onLoadModels: () => void
   onSelectDataset: (datasetId: string) => void
   onSelectModel: (modelId: string) => void
+  onSetTileManifestPath: (value: string) => void
   onSetConfidenceThreshold: (value: number) => void
   onRunSegmentation: () => void
   onLoadRuns: () => void
@@ -52,6 +54,7 @@ export function SegmentationLab({
   segmentationModelError,
   selectedSegmentationDatasetId,
   selectedSegmentationModelId,
+  segmentationTileManifestPath,
   segmentationConfidenceThreshold,
   runningSegmentation,
   segmentationRunResult,
@@ -74,6 +77,7 @@ export function SegmentationLab({
   onLoadModels,
   onSelectDataset,
   onSelectModel,
+  onSetTileManifestPath,
   onSetConfidenceThreshold,
   onRunSegmentation,
   onLoadRuns,
@@ -183,6 +187,15 @@ export function SegmentationLab({
               />
             </label>
           </div>
+          <label>
+            Tile manifest
+            <input
+              type="text"
+              placeholder="Raster tile manifest path"
+              value={segmentationTileManifestPath}
+              onChange={(event) => onSetTileManifestPath(event.target.value)}
+            />
+          </label>
           <button
             className="primary-action"
             type="button"
diff --git a/frontend/src/hooks/useSegmentationWorkflow.ts b/frontend/src/hooks/useSegmentationWorkflow.ts
index 08d568b5..05b27025 100644
--- a/frontend/src/hooks/useSegmentationWorkflow.ts
+++ b/frontend/src/hooks/useSegmentationWorkflow.ts
@@ -31,6 +31,7 @@ export function useSegmentationWorkflow({
   const [segmentationModelError, setSegmentationModelError] = useState<string | null>(null)
   const [selectedSegmentationDatasetId, setSelectedSegmentationDatasetId] = useState('')
   const [selectedSegmentationModelId, setSelectedSegmentationModelId] = useState('segmentation-placeholder')
+  const [segmentationTileManifestPath, setSegmentationTileManifestPath] = useState('')
   const [segmentationConfidenceThreshold, setSegmentationConfidenceThreshold] = useState(0.5)
   const [runningSegmentation, setRunningSegmentation] = useState(false)
   const [segmentationRunResult, setSegmentationRunResult] = useState<SegmentationRunResponse | null>(null)
@@ -137,6 +138,7 @@ export function useSegmentationWorkflow({
         dataset_id: datasetId,
         model_id: selectedSegmentationModelId,
         confidence_threshold: segmentationConfidenceThreshold,
+        tile_manifest_path: segmentationTileManifestPath.trim() || null,
         parameters_json: parameters,
       })
       setSegmentationRunResult(result)
@@ -186,6 +188,7 @@ export function useSegmentationWorkflow({
     setSegmentationItems([])
     setSegmentationGeoJson(null)
     setSegmentationRunResult(null)
+    setSegmentationTileManifestPath('')
   }
 
   return {
@@ -195,6 +198,7 @@ export function useSegmentationWorkflow({
     selectedSegmentationDatasetId,
     selectedSegmentationModelId,
     selectedSegmentationModel,
+    segmentationTileManifestPath,
     segmentationConfidenceThreshold,
     runningSegmentation,
     segmentationRunResult,
@@ -218,6 +222,7 @@ export function useSegmentationWorkflow({
     resetSegmentationForProject,
     setSelectedSegmentationDatasetId,
     setSelectedSegmentationModelId,
+    setSegmentationTileManifestPath,
     setSegmentationConfidenceThreshold,
     setSelectedSegmentationRunId,
     setSegmentationClassFilter,